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The 460 nm measurement was utilized to report reduction of the flavin, while the 540 nm observation was made to track the formation of broad thiolate-FAD charge transfer absorption (Huang et al., 2011

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Aggregating from secondary sources makes your dose rationale harder to defend to ethics committees and reviewers

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Additionally, rectal administration can result in higher bioavailability and faster onset of action compared to some other routes, as the medication bypasses the first-pass metabolism in the liver